New Zealand Journal of Crop and Horticultural Science abstracts
Short communication
In vitro propagation of the PR 204/84 (Prunus persica x
P. amygdalus) rootstock: axillary shoot production and rhizogenesis
Stavros Fotopoulos
Thomas E. Sotiropoulos
National Agricultural Research Foundation
Pomology Institute
P.O. Box 122
59200 Naoussa, Greece
email: thosotir@alfanet.gr
Abstract PR 204/84 rootstock (peach x almond) is a promising
alternative to GF 677 rootstock for peach (Prunus persica) trees. The
present study was the first attempt for its micropropagation. Research was
carried out regarding axillary shoot production and root initiation responses.
6-benzylaminopurine (BAP) at concentrations 2 and 4 µM resulted in a
high yield of axillary shoots in the proliferation stage whereas 6-(g, g-dimethylallylamine)
purine (2-iP) promoted only single shoots. By increasing BAP concentration
from 0.4 to 8 µM, inhibition of shoot growth was observed. By increasing
the levels of indole-3-butyric acid (IBA) and a-naphthaleneacetic acid (NAA)
from 0.05 to 5 µM, a decrease of the proliferation rate of PR 204/84
at all BAP concentrations was observed. The highest shoot length was observed
in the medium containing 4 µM BAP plus 5 µM IBA but it was not
significantly different than 8 µM BAP plus 5µM NAA. Based on
shoot proliferation, shoot length and overall appearance, the best media
of the axillary shoot production study were those with 2 and 4 µM BAP
and the absence of auxins in the medium. After 24 days in culture, excellent
rooting of shoots (100%) was recorded on Murashige and Skoog (MS) media (full
strength) containing 5 and 10 µM IBA and MS media (half strength) containing
2.5, 5, and 10 µM IBA. Mean length of roots was not significantly affected
by IBA and mineral concentration of the culture media. From the results it
was concluded that reducing mineral concentration of MS medium to half the
normal value increased rooting percentage when IBA concentration was 2.5
µM, and mean root number when IBA concentration was 2.5 and 5 µM.
The survival rate of plantlets surviving ex vitro in the greenhouse was 84%.
Keywords callus initiation; indol-3-butyric acid; Prunus
micropropagation; shoot elongation; 6-benzylaminopurine
H04044; Online publication date 8 March 2005 Received 26 April 2004; accepted
21 October 2004
New Zealand Journal of Crop and Horticultural Science, 2005, Vol. 33:
75–79
0014-0671/05/3301-0075 © The Royal Society of New Zealand 2005
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