New Zealand Journal of Crop and Horticultural Science
abstracts
Short communication
Identification of Cucumber mosaic virus in tomato (Lycopersicon esculentum)
growing areas in the north-west Mediterranean region of Turkey
N. Yardimci
H. Eryiğit
Department of Plant Protection
Faculty of Agriculture
Süleyman Demirel University
32260, Isparta, Turkey
email: nejyard@ziraat.sdu.edu.tr
Abstract Leaf samples were collected from 138 tomato (Lycopersicon
esculentum) plants showing symptoms of Cucumber mosaic virus (CMV)
in the north-west Mediterranean region of Turkey. The samples were first tested
by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) using
CMV specific polyclonal antibody. The DAS-ELISA revealed that 53 of the 138
leaf samples tested were infected with CMV. One of the ELISA-positive CMV
isolates was mechanically inoculated into a set of indicator plants by conventional
leaf inoculation method for further characterisation. The virus was multiplied
and showed systemic symptoms in Nicotiana tabacum ‘Xanthii’, Nicotiana
tabacum ‘Samsun NN’, and Capsicum annuum. Double-stranded
RNA (dsRNA) was isolated from uninfected and infected N. tabacum ‘Xanthii’ and C.
annuum plants by CF-11 cellulose chromatography. Agarose gel electrophoresis
showed that CMV-specific dsRNA was present in infected N. tabacum ‘Xanthii’, Nicotiana
tabacum ‘Samsun NN’, and C. annuum plants but not
in healthy plant samples. This result agreed with the biological and serological
findings and confirmed the presence of CMV on tomato growing in the north-west
Mediterranean region of Turkey.
Keywords Cucumber mosaic virus; Lycopersicon esculentum;
double-stranded RNA analysis; ELISA
New Zealand Journal of Crop and Horticultural Science, 2006, Vol. 34:
173–175
0014–0671/06/3402–0173 © The Royal
Society of New Zealand 2006
H05127; Online publication date 27 April 2006. Received 31 October
2005; accepted 15 February 2006
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