New Zealand Journal of Crop and Horticultural Science
abstracts
Plant regeneration via somatic embryogenesis in Farfugium japonicum
Seung-yeob Lee
Institute of Life Science and Natural Resource
Wonkwang University
Iksan, 570-749 Korea
email: sylee@wonkwang.ac.kr
Abstract The leaf and petiole segments of Farfugium japonicum were
cultured on Murashige and Skoog (MS) or Chu N6 media with 2,4-dichlorophenoxyacetic
acid (2,4-D) alone or combinations of auxins and cytokinins for direct or indirect
somatic embryogenesis. Petiole explants produced yellowish embryogenic callus
within 7 weeks on MS medium with 2,4-D alone. Petiole segments were a better
source for embryogenic callus than leaf explants. The frequency of embryogenic
callus and number of somatic embryos per explant were significantly high on
the medium containing 2–5 µM 2,4-D compared to other concentrations.
Direct somatic embryogenesis was observed from petiole segments on N6 medium
containing 5 µM a-naphthalene acetic acid (NAA) and 10 µM kinetin.
When these petiole segments with embryos were transferred on MS medium containing
1 µM NAA and 2 µM kinetin, clusters of secondary
embryos were formed abundantly on the base of the primary somatic embryos.
These clusters subsequently produced numerous plantlets on the MS medium without
plant growth regulators. The survival rate of regenerated plants was 97% after
1 month of transplantation, and they were morphologically similar to the original
plant.
Keywords embryogenic callus; Farfugium japonicum; growth
regulator; plant regeneration; secondary embryo
New Zealand Journal of Crop and Horticultural Science, 2006, Vol. 34:
349–355
0014–0671/06/3404–0349 © The Royal
Society of New Zealand 2006
H06019; Online publication date 20 November 2006. Received 9 March
2006; accepted 22 September 2006
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