New Zealand Journal of Crop and Horticultural Science
abstracts
Effect of desiccation, cold storage, and gibberellic acid on germination
of somatic embryos in walnut (Juglans regia)
Bekir San
Department of Horticulture
Faculty of Agriculture
Suleyman Demirel University
Isparta, Turkey
Hatice Dumanoglu*
Department of Horticulture
Faculty of Agriculture
Ankara University
06110 Ankara, Turkey
email: dmanoglu@agri.ankara.edu.tr
Abstract The effect of desiccation, cold storage, gibberellic acid (GA3 ),
and various combinations of desiccation with GA3 and cold storage
treatments on root formation and germination of walnut (Juglans regia) somatic embryos
originating from immature cotyledons of open-pollinated and non-pollinated
seeds was investigated. Desiccation was effective on germination and cold storage
(4 weeks at 4°C) or GA3 (2.9, 8.6, 14.3, 20.0, and 25.7 µm) treatment
together with desiccation improved the germination rate of walnut somatic embryos.
The highest percentage of germination (69.1%) was determined with desiccated
embryos originating from open-pollinated seeds of ‘Bilecik’ genotype
on ‘Driver’ and ‘Kuniyuki’ walnut (DKW) basal medium
containing 8.6 µm GA3. The germination levels of embryos originating
from both open-pollinated and non-pollinated seeds were found to be similar.
Keywords Juglans regia; root formation; plant regeneration; somatic embryogenesis
New Zealand Journal of Crop and Horticultural Science, 2007, Vol. 35: 73–78
0014–0671/07/3501–0073 © The Royal Society of New Zealand
2007
*Author for correspondence.
H06008; Online publication date 20 March 2007. Received
30 January 2006; accepted 28 August 2006
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