New Zealand Journal of Crop and Horticultural Science abstracts

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Identification of new S-RNase alleles and S-genotype in two pear species (Pyrus pyrifolia and P. bretschneideri) grown in China by genomic PCR and pollination tests

Lin Zhang¹
Xiao-feng Tan^1*
Ta-na Wuyun¹
Jian Qiu¹
De-yi Yuan¹
Qi-rui Wang^1,2
Yu-fen Cao³
Dang-quan Zhang¹

¹ The Key Laboratory of Non-wood Forest Product of Forestry Ministry
Central South University of Forestry and Technology
498 South Shaoshan Road
Changsha City, Hunan 410 004
Peoples’ Republic of China
email: tanxiaofencn@126.com
² Henan Academy of Forestry
Zhengzhou City, Henan 450008
Peoples’ Republic of China
³ Research Institute of Pomology
Chinese Academy of Agricultural Sciences
Xingcheng City, Liaoning 125100
Peoples’ Republic of China

AbstractMost of the economically important Chinese pears display S-RNase-based gametophytic self-incompatibility (GSI). By genomic PCR and sequence analysis, five new S-RNase alleles (S5a and S11 to S14) in three Chinese sand pear (Pyrus pyrifolia) cultivars and six (Sa to Sf) in five Chinese white pear (P. bretschneideri) cultivars were identified. Comparison of nucleotide sequences of S-RNase alleles from the two different Chinese pear species revealed that Sa, Sb,and Sdof P. bretschneideri was identical to S8, S14, and S12 of P. pyrifolia, respectively. We obtained the full-length cDNA sequence of S12 and determined S12 was expressed only in the style of pear. The functions of the new S-RNase alleles were confirmed by the controlled pollination tests. As the results, the eight cultivars of the two Chinese pear species were genotyped as: ‘Maogong’ (S11S12), ‘Cangxixueli’ (S5aS13), ‘Guiguan’ (S2S14), ‘Jinfeng’ (SaSb), ‘Matihuang’ (SbSc), ‘Jinhuasihao’ (SdSe), ‘Youli’ (SbSc), and ‘Jitui’ (ScSf). Elucidation of S-genotype would contribute to higher and stable yields in the orchards and a more efficient breeding programme of pears.

Keywords Chinese pears; self-incompatibility; S-genotype; S-RNases; PCR; 3' RACE; pollination tests

New Zealand Journal of Crop and Horticultural Science, 2007, Vol. 35: 403–413
0014–0671/07/3504–0403 © The Royal Society of New Zealand 2007
*Author for correspondence. H07003; Online publication date 26 October 2007. Received 11 January 2007; accepted 12 August 2007

 

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