New Zealand Journal of Marine and Freshwater Research abstracts

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Development of a field assay for glutamine synthetase, the primary ammonium-assimilating enzyme of cyanobacteria

NIGEL W. KERBY¹

WARWICK F. VINCENT²

¹Agricultural and Food Research Council Research Group on Cyanobacteria Department of Biological Sciences University of Dundee Dundee DD1 4HN, United Kingdom

²Taupo Research Laboratory Department of Scientific and Industrial Research P. O. Box 415, Taupo, New Zealand

Abstract In cyanobacteria, ammonia transported into the cell or formed by N2 fixation or NO3 reduction is assimilated via the primary ammonia-assimilating enzyme glutamine synthetase (GS) (Enzyme Commission No. 6.3.1.2) (see Stewart 1980). GS can be assayed using two techniques, the Mg²⁺-dependent biosynthetic assay:

New Zealand Journal of Marine and Freshwater Research, 1987, Vol. 21: 535-536 OO28-833O/87/21O3-O535$2.5O/O © Crown copyright 1987 Received 21 April 1987; accepted 29 May 1987

PDF file of entire paper: medium quality (177K); (scanned from paper original: notes about this process)

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