New Zealand Journal of Marine and Freshwater Research abstracts
The enzyme cytochemistry of leucocytes in blood and haematopoietic tissues of holocephalans (Chondrichthyes: Chimaeriformes)
P. M. HINE J. M. WAIN
Fisheries Research Centre
Ministry of Agriculture and Fisheries
P.O. Box 297, Wellington, New Zealand
Abstract
Blood films and imprints of orbital lymphomyeloid tissue, spleen, and thymus of six species of holocephalan
(Callorhynchus milii, Chi-maera phantasma, Hydrolagus novaezelandiae, Hydrolagus sp.,
Harriotta raleighana, Rhinochi-maera pacified) were examined for peroxidase, alkaline and acid phosphatases, P-glucuronidase, non-specific and chloroacetate esterases, and periodic acid-Schiff (PAS)-positive substance. Peroxidase was only observed in splenic coarse granulocytes of
Hydrolagus sp. and then it was very weak. Fine granulocytes of all species except
H. raleighana and
R. pacifica stained strongly for alkaline phosphatase, and lymphoid cells in
Hydrolagus sp. were alkaline phosphatase-positive. Acid phosphatase was variable but often weak in all leucocytes. Granulocytes also stained moderately for esterases although coarse granulocytes of some species stained strongly for a-naphthyl acetate ester-ase, but were usually negative or occasionally stained weakly for a-naphthyl butyrate esterase and chloroacetate esterase. In
Hydrolagus sp. and
R. pacifica, cells resembling monocytes stained strongly for a-naphthyl butyrate esterase. In fine granulocyte alkaline phosphatase and moderate esterase content, holocephalans showed more similarity to agnathans than elasmobranchs.
Keywords enzyme cytochemistry; leucocytes; blood; haematopoietic tissues; holocephalans; chimaerids
Received 21 January 1987; accepted 18 June 1987 Fisheries Research Publication 597
New Zealand Journal of Marine and Freshwater Research, 1988, Vol. 22 : 57-62 Crown copyright 1988 Received 21 January 1987; accepted 18 June 1987
PDF file of entire paper: medium quality (979K); (scanned from paper original: notes about this process)
This year's abstracts |
Journal home page |
All abstracts |
Publishing home page
