Wards et al.--Characterisation of _Vibrio ordalii

New Zealand Journal of Marine and Freshwater Research abstracts

Characterisation by restriction endonuclease analysis and plasmid profiling of Vibrio ordalii strains from salmon (Oncorhynchus tshawytscha and Oncorhynchus nerka) with vibriosis in New Zealand

BARRY J. WARDS

HANSAH.PATEL

COLIN D.ANDERSON

GEOFFREY W. de LISLE

Wallaceville Animal Research Centre, MAFQual
Ministry of Agriculture and Fisheries
P. O. Box 40-063, Upper Hutt, New Zealand

Abstract Isolates of Vibrio ordalii were recovered from salmon {Oncorhynchus tshawytscha and Oncorhynchus nerka) cultured in New Zealand. The fish suffered from clinical vibriosis, from seven outbreaks in five geographically distinct areas. Affected fish showed gross signs and histopathology similar to those reported from North America. The presence of a plasmid, designated pVOl, approximately 30 kilobases (kb) in size, was seen in all V. ordalii strains examined. This plasmid was identical in size and restriction endonuclease cleavage analysis to aplasmid (pMJIOl) previously found in V. ordalii strains elsewhere. After tagging pVOl with the transposon Tnl from the R plasmid RP4, attempts to isolate plasmid-less derivatives using a variety of curing agents were unsuccessful. All of the New Zealand isolates possessed identical chromosomal DNA cleavage patterns. These patterns were only slightly different from that of the type strain but substantially different from that of the Vibrio anguillarum type strain.

Keywords Oncorhynchus tshawytscha; Vibrio ordalii; Oncorhynchus nerka; sockeye; quinnat; chinook; Salmonidae; restriction endonuclease analysis; plasmid; vibriosis; Vibrio anguillarum; plasmid curing; Tnl; transposon

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