New Zealand Journal of Marine and Freshwater Research abstracts

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Concentrating Giardia cysts in water by tangential flow filtration compared with centrifugation

JOANNA CHASTE
PHILIP J.KELLY
TIM J. BROWN*

Massey University/New Zealand Health Department Giardia Unit
Department of Microbiology and Genetics School of Biological Sciences, Massey University Private Bag, Palmerston North, New Zealand

Abstract Centrifugation, tangential filtration, and sedimentation were compared as methods of concentrating cyst-containing wash water from filters. A range of cyst concentrations were used on duplicate samples. The cysts were concentrated by one of the above methods and the detectable number of cysts noted. The ability of the methods to process samples with sediment and the overall time taken to process a sample was assessed. The method of choice for the concentration of Giardia cysts in water is centrifugation.

Keywords Giardia cysts; water, concentration; tangential filtration; centrifugation

New Zealand Journal of Marine and Freshwater Research, 1992, Vol. 26: The Royal Society of New Zealand 1992
Received 20 September 1991; accepted 10 April 1992

PDF file of entire paper: medium quality (253K); (scanned from paper original: notes about this process)

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