BOUSTEAD, SHORT-Absence of infectious haematopoietic necrosis ...

New Zealand Journal of Marine and Freshwater Research abstracts

Absence of infectious haematopoietic necrosis virus (IHNV) in New Zealand sockeye salmon, Oncorhynchus nerka

N. C. BOUSTEAD

Freshwater Division
National Institute of Water and Atmospheric
Research Ltd
P. O. Box 8602
Christchurch, New Zealand

T. R. MEYERS S. SHORT

Alaska Department of Fish and Game
Division of Fisheries Rehabilitation, Enhancement
and Development
P. O. Box 25526
Juneau, Alaska 99802-5526
USA

Abstract A study was undertaken of wild and hatchery stocks of fresh water resident sockeye salmon for infectious haematopoietic necrosis virus (IHNV) in New Zealand. From 1988 to 1991, 306 fish were sampled at egg collection from a broodstock reared entirely in captivity. Kidney and some ovarian fluids from 89 wild fish (kokanee) were removed and frozen at -70°C after they spawned in the Waitaki River catchment. Material from the samples was inoculated into tissue culture cell lines of Epithelioma papulosum cyprini (EPC) and chinook salmon embryo (CHSE-214) cells. No IHNV was detected in any of the samples, suggesting that sockeye salmon stocks in New Zealand are free of detectable IHNV.

Keywords Oncorhynchus nerka; sockeye; kokanee; infectious haematopoietic necrosis

New Zealand Journal of Marine and Freshwater Research, 1993: Vol. 27: The Royal Society of New Zealand 1993
Received 9 September 1992; accepted 11 January 1993

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