New Zealand Journal of Marine and Freshwater Research abstracts

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Siderophore production by New Zealand strains of Vibrio anguillarum

VIVIEN PYBUS*
MARGARET W. LOUTIT
JOHN R. TAGG

Department of Microbiology
University of Otago
P.O. Box 56
Dunedin, New Zealand

*Present address: Channing Laboratory, Harvard Medical School, Brigham & Women's Hospital, 180 Longwood Avenue, Boston, MA 02115, U.S.A.

Abstract  New Zealand isolates of Vibrio anguillarum from water, sediments and healthy salmon were tested for their ability to grow under iron-limiting conditions and to produce siderophores. Their growth in the presence of the iron chelator ethylenediamine-di(o-hydroxyphenylacetic acid) (EDDA) suggested they could withstand conditions of iron limitation, with a 200 uM EDDA MIC recorded for the majority of strains. A positive reaction in the chrome azurol sulphate assay after growth in a low iron-containing medium, which was negated when the medium was supplemented with 10 uM iron, indicated they could produce siderophores. The siderophores were of the phenolate class and their biological activities indicated they were related to enterobactin.

Keywords  fish diseases; iron uptake; Vibrio anguillarum; pathogenicity; siderophore; vibriosis

New Zealand Journal of Marine and Freshwater Research, 1994: Vol. 28: 309-315

0028-8330/94/2803-0309 $2.50/0 (c) The Royal Society of New Zealand 1994

PDF file of entire paper: medium quality (574K); (scanned from paper original: notes about this process)

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