New Zealand Journal of Marine and Freshwater Research abstracts

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Induction of triploid embryos by heat shock in the Chilean northern scallop Argopecten purpuratus Lamarck, 1819

JORGE E. TORO
MARIO A. SANHUEZA
LORETO PAREDES
FLAVIA CANELLO

Instituto de Biología Marina
Universidad Austral de Chile
Casilla 567, Valdivia, Chile

Abstract  Triploidy in embryos of the Chilean northern scallop, Argopecten purpuratus Lamarck, 1819, was induced with heat shock. Ploidy levels were assessed by chromosome counting after 3 h from embryos at the 8-16 cell stage of development. The efficiency of induction varied with the time of treatment post-insemination and the temperature of the shock. The duration of the shock showed a positive correlation with the percentage of triploids, the highest of which was obtained using 31deg.C for 15 min applied 10 min after insemination (66.7 %) but with low percentage of survival embryos after 3 h (26.4 %) and 18 days (11.2 %). The optimal procedure for triploidy induction (62.7 %) and survival after 3 h (62 %) and 18 days of culture (30.5 %) was the heat shock of 31deg.C for 10 min applied 10 min after fertilisation. We observed no significant difference in growth of shell length between controls and treated larvae after 18 days of culture.

Keywords  Argopecten purpuratus; triploidy; genetics; Chile

New Zealand Journal of Marine and Freshwater Research, 1995: Vol. 29: 101-105

0028-8330/95/2901-0101 $2.50/0 (c) The Royal Society of New Zealand 1995

Short communication

PDF file of entire paper: medium quality (323K); (scanned from paper original: notes about this process)

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