New Zealand Journal of Marine and Freshwater Research abstracts
Oligonucleotide probe technology as applied to the study
of harmful algal blooms
JOHN V. TYRRELL
PATRICIA R. BERGQUIST+
Centre for Marine Science
School of Biological Sciences
University of Auckland
Private Bag 92 019
Auckland, New Zealand
DAVID J. SAUL
Centre for Gene Technology
School of Biological Sciences
University of Auckland
Private Bag 92 019
Auckland, New Zealand
LINCOLN MACKENZIE
Cawthron Institute
Private Bag 2
Nelson, New Zealand
PETER L. BERGQUIST
School of Biological Sciences
Macquarie University
Sydney, NSW 2109, Australia
and
Department of Molecular Medicine
School of Medicine
University of Auckland
Private Bag 92 019
Auckland, New Zealand
Abstract Harmful algal bloom (HAB) research and monitoring
has traditionally been based on ecological and microbiological measurements
which are laborious, time-consuming, and reliant on experienced operators.
Recent developments in oligonucleotide probe technology and immunofluorescence
research have revealed several potential applications and techniques that may
be transposable to laboratory and field-based monitoring and research. Field
trials are currently underway for fluorescent in situ hybridisation and
sandwich hybridisation assays. The former is particularly suited for
laboratory-based research on harmful algal bloom (HAB) population dynamics and
structure, whereas the sandwich hybridisation assays based on a portable
robotics workstation, offers the potential of quick and reliable laboratory and
possibly field-based screening for HAB species. Initial development is underway
for molecular beacons and the Qß replicase detection system, both offer
the potential of simple and cost effective strategies for field-based
monitoring by people with minimal knowledge of molecular biology.
Keywords oligonucleotide probes; harmful algal blooms;
fluorescent in situ hybridisation; sandwich hybridisation assays; Qß
replicase detection; molecular beacons
New Zealand Journal of Marine and Freshwater Research, 1997, Vol. 31:
551-560
0028-8330/97/3104-0551 $7.00/0 (c) The Royal Society of New Zealand
1997
Review
PDF file of entire paper: medium quality (883K); (scanned from paper original: notes about this process)
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