New Zealand Journal of Marine and Freshwater Research abstracts
Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand
Lesley L. Rhodes1
Joann M. Burkholder2
Howard B. Glasgow2
Parke A. Rublee3
Coy Allen2
Janet E. Adamson1
1Cawthron Institute
Private Bag 2
Nelson, New Zealand
2Center for Applied Aquatic Ecology
North Carolina State University
620 Hutton St., Suite 104
Raleigh
NC 27606, United States
3University of North Carolina at Greensboro
P.O. Box 26 174
Greensboro
NC 27412, United States
Abstract Pfiesteria shumwayae Steidinger et
Burkholder is now known to be present in New Zealand and occurs in estuaries
around the country. The presence of Pfiesteria was initially determined
by a polymerase chain reaction (PCR)-based detection assay, using oligonucleotide
primers targeted at ribosomal DNA extracted from estuarine water and sediments.
Presence was confirmed by isolation from fresh sediments in the presence of
fish (Oreochromis mossambicus), followed by identification by scanning
electron microscopy. The New Zealand isolates of P. shumwayae were
ichthyotoxic in bioassays, but there is no historic evidence of fish kills
in New Zealand associated with the dinoflagellate.
Keywords Pfiesteria; dinoflagellate; estuarine;
fish kills; molecular probes
M02013 Received 20 February 2002; accepted 30 May 2002; published 17 September 2002
New Zealand Journal of Marine and Freshwater Research, 2002, Vol. 36:
621-630
0028-8330/02/3603-0621 $7.00 © The Royal Society of New Zealand
2002
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