New Zealand Journal of Zoology abstracts
Identification primers for sika deer (Cervus nippon) from a
sequence-characterised amplified region (SCAR)
Xiaobing Wu
Hui Liu
College of Life Sciences
Anhui Province Key Laboratory for Conservation and Exploitation of
Biological Resources
Anhui Normal University
Wuhu, 24100
China
Zhigang Jiang*
Institute of Zoology
Chinese Academy of Sciences
Beijing 100080
China
*Author
for correspondence.
Abstract To distinguish sika deer (Cervus nippon)
tissue samples from those of sympatric cervids in the southern part of
Anhui Province, China, we analysed randomly amplified polymorphic DNA
(RAPD) fragments of 12 individual samples from four cervid species:
black muntjac (Muntiacus crinifrons), Reeve’s muntjac (Muntiacus
reevesi), sika deer (Cervus nippon), and tufted deer (Elaphodus
cephalophus). Only one primer among the 100 screened
produced a clear specific band for identifying DNA of sika deer. We
cloned and sequenced 449 bp from this fragment of DNA. We then
designed a pair of 18 bp primers (MHL-U/MHL-D) according to the
sequence, resulting in a 251 bp sequence-characterised amplified
region (SCAR) for sika deer. By combining this pair of SCAR primers
with a universal set of mammalian DNA primers, the identification of
sika deer tissue samples by a simple common multiplex PCR assay
is straightforward, rapid, and reliable. The method will be
useful for cervid conservation and cervid bushmeat trade regulation.
Keywords Cervus nippon; tissue
identification; SCAR; multiplex PCR
Z05005; Received 1 March 2005; accepted 6 October 2005; Online
publication date 24 February 2006
New Zealand Journal of Zoology, 2006, Vol. 33: 65–71
0301–4223/06/3301–0065 © The Royal Society of New Zealand 2006
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